Proteome Profiler™ Antibody Arrays: R&D Systems

Measure More Than 100 Proteins in a Single Sample

No Specialized Equipment Required

What are Proteome Profiler™ Antibody Arrays?

Proteome Profiler Antibody Arrays are simple and economical multiplex assays for early-stage discovery research.

This semiquantitative antibody array allows for the measurement of more than 100 proteins in a single sample. It requires no specialized equipment and eliminates the need for multiple Western blot experiments. Antibody array kits contain buffers, detection antibodies, and membranes spotted in duplicate with high quality capture antibodies. The arrays utilize chemiluminescence for detection data that can be quantified in the same manner as traditional Western blots.

Select antibody arrays are also suitable for use with the LI-COR^® and FluorChem^™ detection systems.

Ready for a more quantitative multiplex assay? Try R&D Systems^® Luminex^® Assays.

Proteome Profiler Target Analytes
Antibody Array Principle
Imaging Systems
Quick Spots Image Analysis Software

Antibody Array Protocol Video
Proteome Profiler Webinar
Additional Resources
Interactive Signaling Pathways

Proteome Profiler Target Analytes

Choose from a wide variety of target analytes, including adipokines, chemokines, cytokines, kinases and proteases.

Multiplex Extracellular Target Analytes

Human, Mouse, and Rat Cytokine Antibody Array

Human, Mouse, and Rat XL Cytokine Antibody Array

Human XL Oncology Antibody Array

Human, Mouse, and Rat Adipokine Antibody Array

Human and Mouse Angiogenesis Antibody Array

Human and Mouse Chemokine Antibody Array

Human Protease Antibody Array

Human Protease Inhibitor Antibody Array

Human Protease/Protease Inhibitor Antibody Array

Human Soluble Receptor Antibody Array, Non-Hematopoietic Panel

Multiplex Intracellular Target Analytes

Human and Mouse Phospho-RTK Antibody Array

Human Phospho-Kinase Antibody Array

Human Cell Stress Antibody Array

Human and Mouse Apoptosis Array

Human Pluripotent Stem Cell Antibody Array

Human Phospho-Immunoreceptor Antibody Array

Human Ubiquitin Antibody Array

Human NFkB Pathway Antibody Array

Did you know? Proteome Profiler Antibody Arrays are amenable to LI-COR near-infrared fluorescence detection.

Antibody Array Principle

Capture antibodies are spotted in duplicate on the nitrocellulose membrane.
Spotted membrane arrays are incubated in a single lysate.
Target analytes are captured by the capture antibody.
Target analytes are detected using a biotinylated or horseradish peroxidase (HRP)-conjugated detection antibody.
Biotinylated detection antibodies are reacted with streptavidin HRP.
Membranes are incubated in a chemiluminescence reagent that produces a light signal proportional to the amount of antibody-target analyte complexes formed.
Data is visualized using standard western blotting methods such as film or digital cameras.

Quick Spots Image Analysis Software

R&D Systems^® now offers Quick Spots image analysis software designed specifically for your Proteome Profiler^™ Arrays.

No more laborious set-up of templates in other imaging software, Quick Spots contains preloaded templates for each Proteome Profiler^™ array. It knows where your results should be and provides the data in less time and with less work.

Download Quick Spots

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Antibody Array Protocol Video

Watch this video to learn how Proteome Profiler Antibody Arrays are used to profile changes in Receptor Tyrosine Kinase Phosphorylation.

Watch the Proteome Profiler Video

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Webinar – Arrays in Cancer Research

Watch this video to learn how Dr. Xin Lu and his research team used Proteome Profiler Antibody Arrays to understand the role that immunosuppressive myeloid cells play in prostate cancer.

Watch Dr. Lu's Webinar